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Experimental and theoretical analysis of a novel deep-bed solid-state bioreactor for cellulolytic enzymes production

Paper ID Volume ID Publish Year Pages File Format Full-Text
3692 182 2011 14 PDF Available
Title
Experimental and theoretical analysis of a novel deep-bed solid-state bioreactor for cellulolytic enzymes production
Abstract

A novel deep-bed solid-state bioreactor was designed and fabricated for cellulolytic enzymes production using mixed fungal cultures. Better temperature and moisture control was achieved through a unique bioreactor design comprising an outer wire-mesh frame with internal air distribution along with near-saturation conditions within the cabinet. Without airflow through the internal distributors, maximum temperatures of 48 °C and 52 °C were observed during half- and full-capacity operation. These were reduced to 44 °C and 43 °C on resumption of airflow. In terms of cellulolytic enzyme production, no significant differences occurred in filter paper activity with depth in half-capacity operation; however, in full-capacity operation, top-level filter paper activity (5.39 FPU/g-solids) was significantly different from middle- and bottom-level activity. Top level beta-glucosidase, endocellulase, and xylanase activities were significantly (P < 0.05) different from middle and bottom levels in both half- and full-capacity operation. A two-phase coupled heat and mass transfer model was developed that predicted the experimental trends reasonably well. Model predictions confirmed that cabinet temperature of 30 °C and distributor airflow rate of 3.42 kg h−1 during operation enabled effective temperature control.

► A novel deep bed solid-state bioreactor was designed and fabricated. ► Performance of bioreactor was evaluated during cellulolytic enzymes production. ► Two-phase heat and mass transfer model was developed. ► Model showed better agreement with observed experimental data. ► Model explicitly predicted the bioreactor performance in various scenarios.

Keywords
Trichoderma reesei; Aspergillus oryzae; Solid-state bioreactor; Cellulolytic enzymes; Heat and mass transfer; N-tank in series model
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Experimental and theoretical analysis of a novel deep-bed solid-state bioreactor for cellulolytic enzymes production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volumes 58–59, 15 December 2011, Pages 110–123
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us