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Electroporation-based applications in biotechnology

Paper ID Volume ID Publish Year Pages File Format Full-Text
36921 45284 2015 9 PDF Available
Title
Electroporation-based applications in biotechnology
Abstract

•We review four promising biotechnological applications of electroporation.•Reversible electroporation is an efficient method for genetic transformation of microorganisms.•Irreversible electroporation allows the inactivation of microbes and the extraction of biomolecules.•Water release due to electroporation increases the efficiency of biomass drying.

Electroporation is already an established technique in several areas of medicine, but many of its biotechnological applications have only started to emerge; we review here some of the most promising. We outline electroporation as a phenomenon and then proceed to applications, first outlining the best established – the use of reversible electroporation for heritable genetic modification of microorganisms (electrotransformation), and then explore recent advances in applying electroporation for inactivation of microorganisms, extraction of biomolecules, and fast drying of biomass. Although these applications often aim to upscale to the industrial and/or clinical level, we also outline some important chip-scale applications of electroporation. We conclude our review with a discussion of the main challenges and future perspectives.

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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 8, August 2015, Pages 480–488
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us