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Towards storage of cells and gametes in dry form

Paper ID Volume ID Publish Year Pages File Format Full-Text
36926 45285 2013 8 PDF Available
Title
Towards storage of cells and gametes in dry form
Abstract

•Liquid nitrogen is the current golden standard for cell and gamete preservation.•Liquid nitrogen storage is effective, but costly, and presents some practical and safety concerns.•Lyophilization has been successfully applied to mammalian spermatozoa.•Dry storage is a promising approach for long-term preservation of cells and gametes.

We review published data on cell/gamete lyophilization. Most studies have utilized the same established protocols for cryopreservation (storage in liquid nitrogen) as for cell lyophilization (dehydration of frozen samples by water sublimation). Surveying natural lyoprotectants, we suggest trehalose and late embryogenesis abundant (LEA) proteins as ideal candidates for the reversible desiccation of mammalian cells/gametes. We find that despite the numerous water subtraction techniques, scientists have relied almost exclusively on lyophilization. There is thus room for improvement in both medium formulation and water subtraction strategies for dry cell/gamete storage. We believe the development of dry processing protocols for use in biobanks of cells/gametes, at reduced cost and with minimal carbon footprint, is within our grasp.

Keywords
lyophilization; liquid nitrogen; anydrobiosis; gametes; long-term storage
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 31, Issue 12, December 2013, Pages 688–695
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us