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ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
36942 45288 2013 9 PDF Available
Title
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Abstract

•ZFNs, TALENs, and CRISPR/Cas-based RNA-guided DNA endonucleases are programmable site-specific nucleases.•Site-specific nucleases induce DNA DSBs that stimulate NHEJ and HDR at targeted genomic loci.•We discuss the therapeutic potential of site-specific nuclease technologies and discuss future prospects for the field.

Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a nonspecific DNA cleavage domain. ZFNs and TALENs enable a broad range of genetic modifications by inducing DNA double-strand breaks that stimulate error-prone nonhomologous end joining or homology-directed repair at specific genomic locations. Here, we review achievements made possible by site-specific nuclease technologies and discuss applications of these reagents for genetic analysis and manipulation. In addition, we highlight the therapeutic potential of ZFNs and TALENs and discuss future prospects for the field, including the emergence of clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases.

Keywords
zinc-finger nuclease; transcription activator-like effector nuclease; clustered regulatory interspaced short palindromic repeat; genome engineering.
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ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 31, Issue 7, July 2013, Pages 397–405
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us