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Electrochemical detection of xanthine in fish meat by xanthine oxidase immobilized on carboxylated multiwalled carbon nanotubes/polyaniline composite film

Paper ID Volume ID Publish Year Pages File Format Full-Text
3696 182 2011 6 PDF Available
Title
Electrochemical detection of xanthine in fish meat by xanthine oxidase immobilized on carboxylated multiwalled carbon nanotubes/polyaniline composite film
Abstract

A commercial xanthine oxidase (XOD) was immobilized covalently onto carboxylated multiwalled carbon nanotubes (c-MWCNT) and polyaniline (PANI) composite film electrodeposited on the surface of a Pt electrode, using N-ethyl-N′-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) chemistry. A xanthine biosensor was fabricated using XOD/c-MWCNT/PANI/Pt electrode as a working electrode, Ag/AgCl (3 M KCl) as standard electrode and Pt wire as auxiliary electrode connected through a potentiostat. The enzyme electrode was characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectrophotometry and electrochemical impedance spectroscopy (EIS). The biosensor showed optimum response within 4 s at pH 7.0 and 35 °C, when polarized at 0.4 V. The optimized xanthine biosensor showed linear response range of 0.6–58 μM, with a detection limit of 0.6 μM (S/N = 3), and a correlation coefficient of 0.98. The biosensor was applied to determine xanthine in fish meat. The biosensor lost 50% of its initial activity after its 200 uses over a period of 100 days.

Graphical abstractA commercial xanthine oxidase (XOD) was immobilized covalently onto carboxylated multiwalled carbon nanotubes (c-MWCNT) and polyaniline (PANI) composite film electrodeposited on the surface of a Pt electrode, using N-ethyl-N′-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) chemistry. The optimized xanthine biosensor showed linear response range of 0.6–58 μM, with a detection limit of 0.6 μM (S/N = 3), and a correlation coefficient of 0.98.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Xanthine; Xanthine oxidase; Electrochemical biosensor; Carboxylated multiwalled carbon nanotubes; Polyaniline; Fish meat
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Electrochemical detection of xanthine in fish meat by xanthine oxidase immobilized on carboxylated multiwalled carbon nanotubes/polyaniline composite film
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volumes 58–59, 15 December 2011, Pages 148–153
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us