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Strategies for improving the physiological relevance of human engineered tissues

Paper ID Volume ID Publish Year Pages File Format Full-Text
36962 45291 2015 7 PDF Available
Title
Strategies for improving the physiological relevance of human engineered tissues
Abstract

•Enhance physiological relevance by using bioreactors, 3D co-cultures, and vascularization.•Long-term culture may be required to generate stable, physiologically relevant tissues.•Nondestructive datapoints are essential in long-term culture for monitoring the same tissue.

This review examines important robust methods for sustained, steady-state, in vitro culture. To achieve ‘physiologically relevant’ tissues in vitro additional complexity must be introduced to provide suitable transport, cell signaling, and matrix support for cells in 3D environments to achieve stable readouts of tissue function. Most tissue engineering systems draw conclusions on tissue functions such as responses to toxins, nutrition, or drugs based on short-term outcomes with in vitro cultures (2–14 days). However, short-term cultures limit insight with physiological relevance because the cells and tissues have not reached a steady-state.

Keywords
long-term culture; tissue engineering; microfluidics; 2D culture; 3D culture; bioreactors
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 7, July 2015, Pages 401–407
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us