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Optical readout of the intracellular environment using nanoparticle transducers

Paper ID Volume ID Publish Year Pages File Format Full-Text
36970 45292 2014 7 PDF Available
Title
Optical readout of the intracellular environment using nanoparticle transducers
Abstract

•Nanoparticle-based transduction provides sub-cellular resolution.•A range of parameters can be probed, including chemical species, pH, and temperature.•These probes can combine imaging, sensing, and targeting in one vehicle.

There is rapid growth in the use of multi-functional nanoparticles as transducers to probe the intracellular environment. New designs of nanoparticles can provide quantitative information at sub-cellular resolution on parameters such as pH, temperature and concentration of nicotinamide adenine dinucleotide (NADH) or selected metal ions. This new work builds on the existing practice of using nanoparticles and fluorescent dyes to provide enhanced microscopic images of cells, but goes beyond it by adding new functionalities and analytical capabilities. In this review, we discuss the recent literature on the development of such nanoparticles for simultaneous biosensing and imaging. We explore and examine the different measurements that will be possible, and analyze the likely accuracy and resolution that could be achieved.

Keywords
intracellular environment; biosensor; nanoparticle; sub-cellular transduction
First Page Preview
Optical readout of the intracellular environment using nanoparticle transducers
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 32, Issue 11, November 2014, Pages 571–577
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering