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Engineering the ligninolytic enzyme consortium

Paper ID Volume ID Publish Year Pages File Format Full-Text
36978 45293 2015 8 PDF Available
Title
Engineering the ligninolytic enzyme consortium
Abstract

•Ligninases are situated at the front line of green chemistry.•Directed ligninase evolution is crucial for emergent lignocellulose biorefineries.•Synthetic secretomes and evolved ancestral ligninases are the next engineering goals.

The ligninolytic enzyme consortium is one of the most-efficient oxidative systems found in nature, playing a pivotal role during wood decay and coal formation. Typically formed by high redox-potential oxidoreductases, this array of enzymes can be used within the emerging lignocellulose biorefineries in processes that range from the production of bioenergy to that of biomaterials. To ensure that these versatile enzymes meet industry standards and needs, they have been subjected to directed evolution and hybrid approaches that surpass the limits imposed by nature. This Opinion article analyzes recent achievements in this field, including the incipient groundbreaking research into the evolution of resurrected enzymes, and the engineering of ligninolytic secretomes to create consolidated bioprocessing microbes with synthetic biology applications.

Keywords
lignin; directed evolution; ligninases; white-rot yeast; ancestral resurrection; Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 3, March 2015, Pages 155–162
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us