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Duplex-specific nuclease-mediated bioanalysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
36981 45293 2015 9 PDF Available
Title
Duplex-specific nuclease-mediated bioanalysis
Abstract

•Duplex-specific nucleases (DSNs) can selectively cleave DNA within RNA/DNA heteroduplexes.•We review the applications of DSNs in biological and biomedicine fields.•DSNs hold promise for the development of amplification-free methods for microRNA detection.•Challenges and perspectives of DSNs are discussed.

Duplex-specific nucleases (DSNs) are promising tools for bioanalysis because of their unique ability to cleave DNA within duplexes while keeping a single strand intact. There is prevalent use of DSNs in both biomedical and biological science applications, such as cDNA library construction, circulating miRNA detection, telomeric overhang detection, and SNP recognition. We present an overview of the current knowledge of DSNs, with special emphasis on DSN-mediated isothermal signal amplification strategies for trace miRNA detection. Continued innovation to address key challenges, such as amplification-free approaches, will open up new avenues in the field of miRNA profiling, offering opportunities for improved personalized medicine, preventive medicine, and translational medicine.

Keywords
duplex-specific nuclease; miRNA; detection; normalization; biosensor; fluorescence assay
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 3, March 2015, Pages 180–188
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us