fulltext.study @t Gmail

Technologies for deriving primary tumor cells for use in personalized cancer therapy

Paper ID Volume ID Publish Year Pages File Format Full-Text
36990 45294 2013 8 PDF Available
Title
Technologies for deriving primary tumor cells for use in personalized cancer therapy
Abstract

•Immortalized cancer cell lines cannot meet the needs of personalized medicine.•Primary cells from solid tumors are important in personalized cancer therapy.•We present pros and cons of different methods for primary tumor cell culture.

For decades, immortal cancer cell lines have constituted an accessible, easily usable set of biological models to investigate cancer biology and explore the potential efficacy of anticancer drugs. However, numerous studies have suggested that these cell lines poorly represent the diversity, heterogeneity, and drug-resistant tumors occurring in patients. The derivation and short-term culture of primary cells from solid tumors have thus gained significant importance in personalized cancer therapy. This review focuses on our current understanding and the pros and cons of different methods for primary tumor cell culture. Furthermore, various culture matrices such as biomimetic scaffolds and chemically defined media supplemented with essential nutrients, have been prepared for different tissues. These well-characterized primary tumor cells redefine cancer therapies with high translational relevance.

Keywords
personalized therapy; primary tumor cell lines
First Page Preview
Technologies for deriving primary tumor cells for use in personalized cancer therapy
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 31, Issue 6, June 2013, Pages 347–354
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering