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Superglue from bacteria: unbreakable bridges for protein nanotechnology

Paper ID Volume ID Publish Year Pages File Format Full-Text
37006 45297 2014 7 PDF Available
Title
Superglue from bacteria: unbreakable bridges for protein nanotechnology
Abstract

•Strong links between proteins are important in many areas of biotechnology.•SpyTag is a peptide forming a spontaneous isopeptide bond to the protein SpyCatcher.•Each component can be fused genetically and reacts under diverse conditions.•Uses include resisting force, targeting nanoparticles, and enhancing enzyme stability.

Biotechnology is often limited by weak interactions. We suggest that an ideal interaction between proteins would be covalent, specific, require addition of only a peptide tag to the protein of interest, and form under a wide range of conditions. Here we summarize peptide tags that are able to form spontaneous amide bonds, based on harnessing reactions of adhesion proteins from the bacterium Streptococcus pyogenes. These include the irreversible peptide–protein interaction of SpyTag with SpyCatcher, as well as irreversible peptide–peptide interactions via SpyLigase. We describe existing applications, including polymerization to enhance cancer cell capture, assembly of living biomaterial, access to diverse protein shapes, and improved enzyme resilience. We also indicate future opportunities for resisting biological force and extending the scope of protein nanotechnology.

Keywords
protein engineering; nanobiotechnology; mechanobiology; synthetic biology; supramolecular assembly; biomimetic
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 32, Issue 10, October 2014, Pages 506–512
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us