fulltext.study @t Gmail

Improvement of cellulose conversion caused by the protection of Tween-80 on the adsorbed cellulase

Paper ID Volume ID Publish Year Pages File Format Full-Text
3706 183 2011 5 PDF Available
Title
Improvement of cellulose conversion caused by the protection of Tween-80 on the adsorbed cellulase
Abstract

For the hydrolysis of pure cellulose, the positive effect of Tween-80 was controversial. In this study, it was found that Tween-80 had little effect on pure cellulose conversion under the shaking speed of 0 and 100 rpm. However, when the shaking speed increased to 180 rpm, Tween-80 resulted in an obvious improvement of cellulose conversion. The mechanism of Tween-80 effect was investigated from three aspects: protecting free cellulase from deactivation, decreasing cellulase proteins adsorption and reducing the cellulase deactivation during the interaction between enzyme and substrate. Tween-80 did not show more positive effects at high speed in protecting free cellulase against deactivation and decreasing cellulase proteins adsorption. From the studies of endoglucanase and exoglucanase relative activities in the supernatant of hydrolysis, it was found that the improved conversion of cellulose with Tween-80 at high shaking speed was mainly due to the reduction of the adsorbed cellulase deactivation resulting from enzyme–substrate interaction.

► Tween-80 can increase the conversion of cellulose at high shaking speed. ► Tween-80 cannot protect free cellulase. ► Tween-80 can decrease adsorption of cellulase proteins. ► Tween-80 can protect the adsorbed cellulase.

Keywords
Tween-80; Cellulase; Enzymatic hydrolysis; Shaking speed; Deactivation; Mechanism
First Page Preview
Improvement of cellulose conversion caused by the protection of Tween-80 on the adsorbed cellulase
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 56, Issue 3, 15 October 2011, Pages 125–129
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us