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Deep mutational scanning: assessing protein function on a massive scale

Paper ID Volume ID Publish Year Pages File Format Full-Text
37081 45309 2011 8 PDF Available
Title
Deep mutational scanning: assessing protein function on a massive scale
Abstract

Analysis of protein mutants is an effective means to understand their function. Protein display is an approach that allows large numbers of mutants of a protein to be selected based on their activity, but only a handful with maximal activity have been traditionally identified for subsequent functional analysis. However, the recent application of high-throughput sequencing (HTS) to protein display and selection has enabled simultaneous assessment of the function of hundreds of thousands of mutants that span the activity range from high to low. Such deep mutational scanning approaches are rapid and inexpensive with the potential for broad utility. In this review, we discuss the emergence of deep mutational scanning, the challenges associated with its use and some of its exciting applications.

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Deep mutational scanning: assessing protein function on a massive scale
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 29, Issue 9, September 2011, Pages 435–442
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us