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Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase

Paper ID Volume ID Publish Year Pages File Format Full-Text
3709 183 2011 8 PDF Available
Title
Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase
Abstract

The super paramagnetic nanoparticles Fe3O4/(2-hydroxyethty methacrylate-co-dimethyl dially ammonium chloride) copolymer, i.e. Fe3O4/P(HEMA–DMDAAC), with positive charge and active epoxy groups simultaneously, were synthesized by a surface-initiated radical polymerization and activation reaction with epoxy chloropropane. The production particles were characterized by Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TG), transmission electron microscopy (TEM), and vibrating sample magnetometry (VSM). It was verified that the magnetic microspheres held small diameters of 80–100 nm and displayed super paramagnetic property with saturation magnetization of 38.9 emu/g. The candida rugosa lipase (CRL), meanwhile, was immobilized onto the magnetic microspheres via electrostatic adsorption and covalent binding, the loading amount of lipase was 68.3 ± 0.5 mg CRL/g support and the activity recovery of the obtained immobilized lipase reached to 60.4% (±1.6%).

► Super paramagnetic nanoparticles were prepared by a surface-initiated radical polymerization. ► This nanoparticle was provided with positive charge and active epoxy groups simultaneously. ► The candida rugosa lipase (CRL) was immobilized via electrostatic adsorption and covalent binding. ► The lipase loading amount was 68.3 ± 0.5 mg CRL/g support and the activity recovery reached to 60.4% (±1.6%).

Keywords
Super paramagnetism; Electrostatic adsorption; Covalent binding; Immobilized CRL; Enzyme technology; Reusability
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Preparation of carriers based on magnetic nanoparticles grafted polymer and immobilization for lipase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 56, Issue 3, 15 October 2011, Pages 142–149
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us