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Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts

Paper ID Volume ID Publish Year Pages File Format Full-Text
37127 45313 2013 10 PDF Available
Title
Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts
Abstract

Enzyme immobilization on solid supports has been key to biotransformation development. Although technologies for immobilization have largely reached maturity, the resulting biocatalysts are not well understood mechanistically. One limitation is that their internal environment is usually inferred from external data. Therefore, biological consequences of the immobilization remain masked by physical effects of mass transfer, obstructing further development. Work reviewed herein shows that opto-chemical sensing performed directly within the solid support enables the biocatalyst's internal environment to be uncovered quantitatively and in real time. Non-invasive methods of intraparticle pH and O2 determination are presented, and their use as process analytical tools for development of heterogeneous biocatalysts is described. Method diversification to other analytes remains a challenging task for the future.

Keywords
biocatalysis; immobilization; solid support; internal sensing and monitoring; intraparticle pH and O2 concentration; process analytical technology
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Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 31, Issue 3, March 2013, Pages 194–203
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us