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Monitoring protein interactions and dynamics with solvatochromic fluorophores

Paper ID Volume ID Publish Year Pages File Format Full-Text
37140 45315 2010 11 PDF Available
Title
Monitoring protein interactions and dynamics with solvatochromic fluorophores
Abstract

Solvatochromic fluorophores possess emission properties that are sensitive to the nature of the local microenvironment. These dyes have been exploited in applications ranging from the study of protein structural dynamics to the detection of protein-binding interactions. Although the solvatochromic indole fluorophore of tryptophan has been utilized extensively for in vitro studies to advance our understanding of basic protein biochemistry, the emergence of new extrinsic synthetic dyes with improved properties, in conjunction with recent developments in site-selective methods to incorporate these chemical tools into proteins, now open the way for studies in more complex systems. Herein, we discuss recent technological advancements and their application in the design of powerful reporters, which serve critical roles in modern cell biology and assay development.

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Monitoring protein interactions and dynamics with solvatochromic fluorophores
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 28, Issue 2, February 2010, Pages 73–83
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering