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Permanent genome modifications in plant cells by transient viral vectors

Paper ID Volume ID Publish Year Pages File Format Full-Text
37145 45316 2011 7 PDF Available
Title
Permanent genome modifications in plant cells by transient viral vectors
Abstract

Endonuclease-mediated induction of genomic double-strand breaks has enabled genome editing in living cells. However, deploying this technology for the induction of gene disruption in plant cells often relies on direct gene transfer of endonuclease (i.e. zinc finger nuclease or homing endonuclease) expression constructs into the targeted cell, followed by regeneration of a mutated plant. Such mutants, even when they have no detectable traces of foreign DNA, might still be classified as transgenic because of the transgenic nature of the endonuclease delivery method. Indirect delivery of endonucleases into target cells by viral vectors provides a unique non-transgenic approach to the production of mutated plants. Furthermore, viral vectors can spread into the growing and developing tissues of infected plants, which could provide a unique opportunity to bypass the regeneration step that is often required in direct gene-transfer methods.

First Page Preview
Permanent genome modifications in plant cells by transient viral vectors
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 29, Issue 8, August 2011, Pages 363–369
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering