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Decorating microbes: surface display of proteins on Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
37165 45318 2011 8 PDF Available
Title
Decorating microbes: surface display of proteins on Escherichia coli
Abstract

Bacterial surface display entails the presentation of recombinant proteins or peptides on the surface of bacterial cells. Escherichia coli is the most frequently used bacterial host for surface display and, as such, a variety of E. coli display systems have been described that primarily promote the surface exposure of peptides and small proteins. By contrast, display systems based on autotransporter proteins (ATs) and ice nucleation protein (INP) are excellent systems for the display of large and complex proteins, and are therefore of considerable biotechnological relevance. Here, we review recent advances in AT and INP-mediated display and their biotechnological applications. Additionally, we discuss several promising alternative display methods, as well as novel bacterial host organisms.

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Decorating microbes: surface display of proteins on Escherichia coli
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 29, Issue 2, February 2011, Pages 79–86
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering