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Efficient Agaricus bisporus tyrosinase immobilization on cellulose-based carriers

Paper ID Volume ID Publish Year Pages File Format Full-Text
3721 183 2011 9 PDF Available
Title
Efficient Agaricus bisporus tyrosinase immobilization on cellulose-based carriers
Abstract

A systematic study of the covalent immobilization of Agaricus bisporus tyrosinase on man-tailored cellulose-based carriers with different pore structure and functionalization showed that DEAE-Granocel with the largest mean pore diameter and activated with divinyl sulphone can offer highly active preparations with a monophenolase (L-Tyrosine) and diphenolase (L-DOPA) activity immobilization efficiency of over 34% and 24%, respectively. A thermal stability test carried out at 55 °C indicated monophasic activity depletion with the inactivation rate of immobilized enzyme of 0.021 min−1 and 22 times higher (0.454 min−1) for the native enzyme. The susceptibility of native and immobilized tyrosinase to suicide inactivation in the presence of monophenolic (L-Tyrosine, phenol, p-chlorophenol, p-cresol, p-aminophenol, o-aminophenol) and diphenolic (L-DOPA, catechol, p-tert-butylcatechol) substrates was tested in repeated batch tests and was found to be critical for obtaining stable biocatalysts in reactions with diphenolic substrates.

► We investigated tyrosinase immobilization on synthesized cellulose-based carriers. ► Covalently bound enzyme was 22 times more stable than native tyrosinase. ► Operational stability was tested with 6 monophenolic and 3 diphenolic substrates. ► Repeated batch tests confirmed suicide inactivation caused by diphenolic substrates.

Keywords
Tyrosinase; Immobilization; Cellulose carrier; Stability; Batch reactor; Operational stability
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Efficient Agaricus bisporus tyrosinase immobilization on cellulose-based carriers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 56, Issue 3, 15 October 2011, Pages 232–240
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us