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Cellulases for biomass degradation: comparing recombinant cellulase expression platforms

Paper ID Volume ID Publish Year Pages File Format Full-Text
37241 45327 2013 13 PDF Available
Title
Cellulases for biomass degradation: comparing recombinant cellulase expression platforms
Abstract

•Each organism has different strengths and weaknesses in recombinant glycosyl hydrolase (GH) expression.•Advances using yeasts and plants show particularly great promise.•Advancements in enzyme surface display, and the creation of chimeras and cellulosomes.•Approaches using multiple strategies of GH expression may be desirable.

Improvement of cellulase expression has the potential to change the nature of the biofuel industry. Increasing the economic feasibility of cellulase systems would significantly broaden the range of practicable biomass conversion, lowering the environmental impact of our civilisations’ fuel needs. Cellulases are derived from certain fungi and bacteria, which are often difficult to culture on an industrial scale. Accordingly, methods to recombinantly express important cellulases and other glycosyl hydrolase (GH) enzymes are under serious investigation. Herein, we examine the latest developments in bacterial, yeast, plant, and fungal expression systems. We discuss current strategies for producing cellulases, and evaluate the benefits and drawbacks in yield, stability, and activity of enzymes from each system, and the overall progress in the field.

Keywords
glycosyl hydrolase; heterologous expression; synthetic biology; consolidated bioprocessing; cellulolytic activity; cellulosome; biomass; industrial processing
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Cellulases for biomass degradation: comparing recombinant cellulase expression platforms
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 31, Issue 10, October 2013, Pages 581–593
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us