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PEGylated liposomes encapsulating human hemoglobin enhance oxygen transfer and cell proliferation while decreasing cell hypoxia in fibrin

Paper ID Volume ID Publish Year Pages File Format Full-Text
3727 184 2011 7 PDF Available
Title
PEGylated liposomes encapsulating human hemoglobin enhance oxygen transfer and cell proliferation while decreasing cell hypoxia in fibrin
Abstract

Oxygen supply to cells in three-dimensional cultures occurs mainly by diffusion, with insufficient oxygen concentration reaching the center of cell aggregates, thus creating hypoxic areas and leading eventually to cell death. The aims of this study were, firstly to measure and characterize the oxygen transfer from liposomes encapsulating human hemoglobin (LEH) to an aqueous environment and, secondly, to study the effects of LEH to cells grown in a three-dimensional environment. LEH were loaded with pure oxygen and the oxygen transfer from the liposomes to an aqueous phase was monitored. Human umbilical vein endothelial cells (HUVEC) were cultured in a fibrin gel mimicking a diffusion-limited environment. After 24 h, HUVEC seeded in fibrin covered with culture medium supplemented with oxygen-loaded LEH showed a decrease in HIF-1α expression and an increase in proliferation compared to those in fibrin cultured either with medium containing liposomes with no hemoglobin or non-modified medium.

► Liposomes encapsulating hemoglobin enhance oxygen transfer. ► Liposomes encapsulating hemoglobin enhance cell proliferation in fibrin. ► Liposomes encapsulating hemoglobin reduce HIF-1α (hypoxia) expression in cells grown in fibrin.

Keywords
Hypoxia; Oxygen carriers; Liposomes encapsulating hemoglobin; Fibrin; Endothelial cells; Oxygen transfer
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PEGylated liposomes encapsulating human hemoglobin enhance oxygen transfer and cell proliferation while decreasing cell hypoxia in fibrin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 55, Issue 3, 15 August 2011, Pages 162–168
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us