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New advances in production and functional folding of G-protein-coupled receptors

Paper ID Volume ID Publish Year Pages File Format Full-Text
37309 45338 2011 9 PDF Available
Title
New advances in production and functional folding of G-protein-coupled receptors
Abstract

G-protein-coupled receptors (GPCRs), the largest family of integral membrane proteins, participate in the regulation of many physiological functions and are the targets of approximately 30% of currently marketed drugs. However, knowledge of the structural and molecular bases of GPCR functions remains limited owing to difficulties related to their overexpression, purification and stabilization. The development of new strategies aimed at obtaining large amounts of functional GPCRs is therefore crucial. Here, we review the most recent advances in the production and functional folding of GPCRs from Escherichia coli inclusion bodies. Major breakthroughs open exciting perspectives for structural and dynamic investigations of GPCRs. In particular, combining targeting to bacterial inclusion bodies with amphipol-assisted folding is emerging as a highly powerful strategy.

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New advances in production and functional folding of G-protein-coupled receptors
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 29, Issue 7, July 2011, Pages 314–322
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us