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Zinc finger nuclease technology heralds a new era in mammalian transgenesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
37318 45339 2010 8 PDF Available
Title
Zinc finger nuclease technology heralds a new era in mammalian transgenesis
Abstract

Non-mouse mammalian transgenesis is limited by its overall inefficiency and technical hurdles. Recent years have seen the emergence of two approaches that are applicable to most mammals. The first, based on lentivirus vectors, allows efficient generation of transgenic founders, most of them expressing the transgene. The second, recently applied to produce transgenic fish and mammals, takes advantage of the design of specific ‘DNA-scissors’ for efficient introduction of subtle mutations in potentially any region of the genome. This review focuses on the potential of this latter technology to modify mammalian genomes without the need to apply challenging and less-efficient protocols. We highlight the complementary aims of these new approaches and the as-yet-unexplored possibilities offered by their combination.

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Zinc finger nuclease technology heralds a new era in mammalian transgenesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 28, Issue 3, March 2010, Pages 134–141
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us