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Selective partition of plasmid DNA and RNA from crude Escherichia coli cell lysate by aqueous two-phase systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
3736 184 2011 3 PDF Available
Title
Selective partition of plasmid DNA and RNA from crude Escherichia coli cell lysate by aqueous two-phase systems
Abstract

In this paper, the partition of plasmid DNA (pDNA) and RNA in polyethylene glycol (PEG) and di-potassium phosphate aqueous two-phase systems (ATPS) by adding up of NaCl salt was studied with crude Escherichia coli (E. coli) cell lysate. The partition of nucleic acid was studied in an ATPS composed of 17% (w/w) PEG300, 14% (w/w) di-potassium phosphate (designated as 17/14) and 40% (w/w) lysate. It is demonstrated that in a 17/14 system with 40% (w/w) lysate, at least 2% (w/w) or 342 mM (kg ATPS)−1 NaCl were able to selective partition the pDNA to the bottom phase.

► The application of PEG-phosphate ATPS could be extended to the selective removal of RNA from pDNA in crude cell lysate. ► It is demonstrated that in a 17/14 system with 40% (w/w) lysate, at least 2% (w/w) or 342 mM (kg ATPS)−1 NaCl were required to partition pDNA to the bottom phase.

Keywords
Bioseparations; Aqueous two-phase system; Purification; Integrated processing; RNA; DNA
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Selective partition of plasmid DNA and RNA from crude Escherichia coli cell lysate by aqueous two-phase systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 55, Issue 3, 15 August 2011, Pages 230–232
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us