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Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
37405 45350 2008 8 PDF Available
Title
Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA
Abstract

The diversity of biomolecules present in plasmid DNA (pDNA)-containing extracts and the structural and chemical similarities between pDNA and impurities are some of the main challenges of improving or establishing novel purification procedures. In view of the unequalled specificity of affinity purification, this technique has recently begun to be applied in downstream processing of plasmids. This paper discusses the progress and importance of affinity chromatography (AC) for the purification of pDNA-based therapeutic products. Several affinity approaches have already been successfully developed for a variety of applications, and we will focus here on highlighting their possible contributions to the pDNA purification challenge. Diverse affinity applications and their advantages and disadvantages are discussed, as well as the most significant results and improvements in the challenging task of purifying plasmids.

First Page Preview
Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 26, Issue 9, September 2008, Pages 518–525
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering