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Comparison of substance supply in static and perfusion cultures based on mass transport phenomena

Paper ID Volume ID Publish Year Pages File Format Full-Text
3748 185 2011 6 PDF Available
Title
Comparison of substance supply in static and perfusion cultures based on mass transport phenomena
Abstract

Mass transport phenomena in cell culture can be formulated by using classical reaction-diffusion equations; however, in practice, it is difficult to solve these equations analytically. Here, we used computer simulation to solve these equations, and compared the time-dependent concentration profile of substances in conventional static culture with that in perfusion culture. The simulated glucose consumption in static culture agreed with that of actual culture conditions used in a general cell culture experiment. The simulation of perfusion culture revealed that the geometry of the chamber and its operating parameters are critical to obtaining a sufficient supply of substances. We also found that the previously reported perfusion chambers are well designed and operate under adequate conditions. Our kinetic model of time-dependent concentration profiles for general substances based on mass transport phenomena could, therefore, be used for the optimal design of a microfluidic perfusion culture chip.

► Mathematical modeling was used to assess substance supply in cell cultures. ► Simulation of static culture conditions resembles actual culture conditions. ► Simulation was used to evaluate previously reported perfusion chambers. ► The mathematical model could be use to design a microfluidic cell culture chip.

Keywords
Mass transfer; Tissue cell culture; Bioprocess design; Oxygen transfer; Static culture; Microfluidic perfusion culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 57, 15 November 2011, Pages 69–74
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us