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The forgotten variables of DNA array hybridization

Paper ID Volume ID Publish Year Pages File Format Full-Text
37528 45366 2006 6 PDF Available
Title
The forgotten variables of DNA array hybridization
Abstract

The reproducibility and reliability of DNA array measurements have been repeatedly questioned during the years. A reassessment of fundamental variables of nucleic acid hybridization might help to solve some of these problems. The hybridization equilibrium, t1/2, is 41 days for a target human genome. Similar hybridization t1/2 are expected for whole-transcriptome chips hybridized with tissue cDNA. This implies that most studies on mammalian cells have not been performed under equilibrium conditions. Non-equilibrium binding introduces a stochastic factor into hybridization dynamics; in other words, hybridization will prevail at different spots in different experiments, everything else being equal. A careful re-evaluation of results obtained under non-equilibrium conditions might prove fruitful and help to explain instances where findings conflict.

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The forgotten variables of DNA array hybridization
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 24, Issue 10, October 2006, Pages 443–448
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us