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How to overcome limitations in biotechnological processes - examples from hydroxynitrile lyase applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
37547 45368 2009 9 PDF Available
Title
How to overcome limitations in biotechnological processes - examples from hydroxynitrile lyase applications
Abstract

During the last decades, enzymes became very versatile catalysts for a variety of reactions including natural and unnatural compounds. However, many enzyme-catalysed reactions suffer from diverse restrictions because of limitations related to process parameters or the enzyme. The understanding and overcoming of those undesired side effects is therefore mandatory for the implementation of optimal process parameters. To achieve this aim, various methods from molecular biology and reaction engineering can be employed. By focusing on the hydroxynitrile lyase-catalysed synthesis of enantiopure cyanohydrins, we give an overview of strategies to improve commercially utilized enzymes and to suppress non-enzymatic reactions. Particular emphasis is placed on the necessity to combine approaches from different fields, such as enzyme engineering and reaction engineering.

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How to overcome limitations in biotechnological processes - examples from hydroxynitrile lyase applications
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 27, Issue 10, October 2009, Pages 599–607
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us