fulltext.study @t Gmail

A new tool for biotechnology: AdoMet-dependent methyltransferases

Paper ID Volume ID Publish Year Pages File Format Full-Text
37605 45375 2007 6 PDF Available
Title
A new tool for biotechnology: AdoMet-dependent methyltransferases
Abstract

AdoMet-dependent methyltransferases catalyze highly specific methyl group transfers from the ubiquitous cofactor S-adenosyl-l-methionine to a multitude of biological targets in the cell. Recently, DNA methyltransferases have been used for the sequence-specific, covalent attachment of larger chemical groups to plasmid and bacteriophage DNA using two classes of synthetic AdoMet analogs. These synthetic cofactors, in combination with the myriad AdoMet-dependent methyltransferases available in nature, provide new molecular tools for precise, targeted functionalization and labeling of large natural DNAs and, in all likelihood, RNAs and proteins. This paves the way for numerous novel applications in the functional analysis of biological methylation, biotechnology and medical diagnostics.

First Page Preview
A new tool for biotechnology: AdoMet-dependent methyltransferases
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 25, Issue 3, March 2007, Pages 99–104
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering