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Expanding the metabolic engineering toolbox: more options to engineer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
37610 45375 2007 6 PDF Available
Title
Expanding the metabolic engineering toolbox: more options to engineer cells
Abstract

Metabolic engineering exploits an integrated, systems-level approach for optimizing a desired cellular property or phenotype; and great strides have been made within this scope and context during the past fifteen years. However, due to limitations in the concepts and techniques, these have relied on a focused, pathway-oriented view. Recent advances in ‘omics’ technologies and computational systems biology have brought the foundational systems approach of metabolic engineering into focus. At the same time, protein engineering and synthetic biology have expanded the breadth and precision of the methods available to metabolic engineers to improve strain properties. Examples are presented that illustrate this broader perspective of tools and concepts, including a recent approach for global transcriptional machinery engineering (gTME), which has demonstrated the ability to elicit multigenic transcriptional changes that have improved phenotypes compared with single-gene perturbations.

First Page Preview
Expanding the metabolic engineering toolbox: more options to engineer cells
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 25, Issue 3, March 2007, Pages 132–137
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering