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Homogeneous versus heterogeneous probes for microbial ecological microarrays

Paper ID Volume ID Publish Year Pages File Format Full-Text
37617 45376 2006 6 PDF Available
Title
Homogeneous versus heterogeneous probes for microbial ecological microarrays
Abstract

Microbial ecological microarrays have been developed for investigating the composition and functions of microorganism communities in environmental niches. These arrays include microbial identification microarrays, which use oligonucleotides, gene fragments or microbial genomes as probes. In this article, the advantages and disadvantages of each type of probe are reviewed. Oligonucleotide probes are currently useful for probing uncultivated bacteria that are not amenable to gene fragment probing, whereas the functional gene fragments amplified randomly from microbial genomes require phylogenetic and hierarchical categorization before use as microbial identification probes, despite their high resolution for both specificity and sensitivity. Until more bacteria are sequenced and gene fragment probes are thoroughly validated, heterogeneous bacterial genome probes will provide a simple, sensitive and quantitative tool for exploring the ecosystem structure.

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Homogeneous versus heterogeneous probes for microbial ecological microarrays
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 24, Issue 7, July 2006, Pages 318–323
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us