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A facile pretreatment method for efficient immobilization of penicillin G acylase

Paper ID Volume ID Publish Year Pages File Format Full-Text
3764 187 2011 6 PDF Available
Title
A facile pretreatment method for efficient immobilization of penicillin G acylase
Abstract

A facile pretreatment method was developed to obtain high specific activity of PGA from recombinant E. coli cells for preparing immobilized enzyme with high activity, which coupled selective extraction of PGA with butyl acetate and adsorption of butyl acetate with active carbon. Butyl acetate (5%, v/v) led to a 92.0% release of PGA, the specific activity of which in the extracting solution was twice that obtained by sonication. The negative effect on PGA immobilization due to residual butyl acetate in the extracting solution was removed by adding active carbon (8%, w/v). The final PGA solution meets requirement for industrial immobilization while eliminating the high cost of a traditional purification process. This pretreatment method developed in this work is simple, highly performing and cost-effective. It has been employed in a 10 tons/year immobilized PGA production line.

Keywords
Penicillin G acylase (PGA); Butyl acetate; Extraction; Active carbon; Aminated carrier; Immobilization
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A facile pretreatment method for efficient immobilization of penicillin G acylase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 56, Issues 1–2, 15 September 2011, Pages 17–22
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us