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E unum pluribus: multiple proteins from a self-processing polyprotein

Paper ID Volume ID Publish Year Pages File Format Full-Text
37644 45379 2006 8 PDF Available
Title
E unum pluribus: multiple proteins from a self-processing polyprotein
Abstract

Many applications of genetic engineering require transformation with multiple (trans)genes, although to achieve these using conventional techniques can be challenging. The 2A oligopeptide is emerging as a highly effective new tool for the facile co-expression of multiple proteins in a single transformation step, whereby a gene encoding multiple proteins, linked by 2A sequences, is transcribed from a single promoter. The polyprotein self-processes co-translationally such that each constituent protein is generated as a discrete translation product. 2A functions in all the eukaryotic systems tested to date and has already been applied, with great success, to a broad range of biotechnological applications: from plant metabolome engineering to the expression of T-cell receptor complexes, monoclonal antibodies or heterodimeric cytokines in animals.

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E unum pluribus: multiple proteins from a self-processing polyprotein
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 24, Issue 2, February 2006, Pages 68–75
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us