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Process optimization for the production of rhamnolipid and formation of biofilm by Pseudomonas aeruginosa CPCL on polypropylene

Paper ID Volume ID Publish Year Pages File Format Full-Text
3766 187 2011 9 PDF Available
Title
Process optimization for the production of rhamnolipid and formation of biofilm by Pseudomonas aeruginosa CPCL on polypropylene
Abstract

The effect of rhamnolipid during the formation of initial biofilm (48 h) by Pseudomonas aeruginosa CPCL on polypropylene surface was studied. This strain was isolated from a petroleum contaminated soil and it produces a mixture of rhamnolipids. The nutritional and environmental conditions necessary for optimal production of rhamnolipid, attachment of rhamnolipid and formation of thicker biofilm on the polypropylene surface were found to be different from one another. Comparatively lower pH and temperature, higher concentration of ammonium chloride and rotation velocity of the shaker were required for the biofilm formation and the attachment of rhamnolipid on the polypropylene surface. Screening design showed that the extent of viable cells and rhamnolipid attached to the polypropylene surface was positively correlated (r = 0.474; p < 0.01). However, central composite design did not show any such relationship, possibly because of the different experimental ranges used in central composite design. There were considerable variations in the thickness and spatial arrangement of biofilms grown under these three different optimized conditions. Higher substratum coverage and thicker biofilm were observed under the condition optimized for biofilm formation when compared to the other two conditions. This study is essential since P. aeruginosa biofilms are found to have implication in bioremediation of recalcitrant pollutants.

► Optimized the conditions for thicker biofilm formation by P. aeruginosa CPCL on PP. ► Increase in the concentration of glucose favours thicker biofilm formation. ► Temperature has negative effect on the formation of thicker biofilm. ► Shear stress has no effect on the formation of biofilm by P. aeruginosa on PP. ► Rhamnolipid regulates the spatial arrangement of the biofilm.

Keywords
Fractional factorial design; Rhamnolipid; Biofilm; Polypropylene; Central composite design
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Process optimization for the production of rhamnolipid and formation of biofilm by Pseudomonas aeruginosa CPCL on polypropylene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 56, Issues 1–2, 15 September 2011, Pages 37–45
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us