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Enhancement of enterocin A production by Enterococcus faecium MMRA and determination of its stability to temperature and pH

Paper ID Volume ID Publish Year Pages File Format Full-Text
3773 187 2011 13 PDF Available
Title
Enhancement of enterocin A production by Enterococcus faecium MMRA and determination of its stability to temperature and pH
Abstract

The growth, nutrient (total sugars, phosphorous, nitrogen and proteins) consumption and product formation by Enterococcus faecium MMRA in de Man, Rogosa and Sharpe (MRS) broth was followed in non-realkalized batch, realkalized batch and realkalized fed-batch cultures. In the latter fermentation, the growing culture was fed with a mixture of MRS medium (20 g of glucose/L) and a 400 g/L concentrated glucose. In the three cultures, a typically homolactic fermentation (only lactic acid was produced) was observed. The realkalized fed-batch culture was mainly characterized with higher biomass (2.4 g/L), lactic acid (32.5 g/L) and enterocin A (35.9 AU/mL) concentrations compared with the two batch processes.Mathematical models were developed to describe the productions of biomass, enterocin A and lactic acid in the two realkalized cultures. The growth and lactic acid production were successfully modelled with the Monod and the Luedeking and Piret models, respectively. Enterocin A was modelled by using a modified form of the Luedeking and Piret model, which includes a term for the effect of the pH drop rate on bacteriocin synthesis. Furthermore, the maximum enterocin A stability was obtained at temperatures below 100 °C, at acidic pH values and short incubation times.

► Enterocin A synthesis was assayed in MRS broth in batch and fed-batch fermentations. ► The realkalized fed-batch fermentation technique enhanced enterocin A synthesis. ► The enterocin A production system in this culture was satisfactorily modelled. ► This bacteriocin was classified as a primary metabolite dependent on the pH drop. ► Bacteriocin stability was high around 84 °C, at low pH, and short incubation times.

Keywords
Enterococcus faecium MMRA; Enterocin A; Realkalized batch; Fed-batch; Fermentation; Modelling; Temperature; pH
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 56, Issues 1–2, 15 September 2011, Pages 94–106
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us