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Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
37733 45400 2006 6 PDF Available
Title
Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells
Abstract

Recombinant proteins form an increasingly large part of the portfolio of biopharmaceutical companies. Production of these often complex transgenic proteins is achieved predominantly in mammalian cell lines but the process is hampered by low yields and unstable expression. Some of these problems are caused by gene silencing at the level of chromatin – so-called epigenetic gene silencing. Here, we describe approaches, which have emerged during the past few years, designed to interfere with epigenetic gene silencing with the aim of enhancing and stabilizing transgene expression. These include targeting histones, the inclusion of specific DNA elements and targeting sites of high gene-expression. We conclude that employing epigenetic gene regulation tools, in combination with further process optimization, might represent the next step forward in the production of therapeutic proteins.

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Employing epigenetics to augment the expression of therapeutic proteins in mammalian cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 24, Issue 3, March 2006, Pages 137–142
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us