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Expression, purification of a novel alkaline Staphylococcus xylosus lipase acting at high temperature

Paper ID Volume ID Publish Year Pages File Format Full-Text
3778 188 2011 10 PDF Available
Title
Expression, purification of a novel alkaline Staphylococcus xylosus lipase acting at high temperature
Abstract

A new Staphylococcus xylosus strain was isolated. The extracellular lipase of S. xylosus (wt-SXL2) was purified to homogeneity from the culture medium. The specific activity of the purified enzyme, measured at pH 8.5 and 55 °C using tributyrin or olive oil emulsion, reached, respectively, 6300 U/mg or 2850 U/mg. The sequenced 18 N-terminal amino acid showed a high degree of identity with known staphylococcal lipase sequences.The gene encoding the mature lipase was cloned and sequenced. The deduced amino acid sequence showed a significant similarity with various staphylococcal lipases. The highest overall identity (98.74%) was found with S. xylosus lipase (SXL1). The mature part of the lipase was expressed in Escherichia coli. The recombinant lipase was purified by affinity chromatography. The specific activity of the recombinant lipase was 4100 or 1500 U/mg using tributyrin or olive oil emulsion as substrate, respectively, at pH 8.5 and 55 °C.The wild type and recombinant lipases presented a quite interesting thermal stability, after an incubation of 60 min at 55 °C and they are found to be highly stable at a pH ranging from 4 to 11. Due to its stability at high temperature and in organic solvent, the wt-SXL2 was used as biocatalyst to synthesise a high added value molecules.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights► A newly isolated Staphylococcus xylosus produce a thermostable and alkaline lipase. ► The Staphylococcus xylosus lipase is the most active lipase in the staphylococcal family. ► The immobilized SXL2 was used as biocatalyst to synthesise a high added value molecules.

Keywords
Wt-SXL2, Staphylococcus xylosus lipase 2; SSL, Staphylococcus simulans lipase; SXL1, Staphylococcus xylosus lipase 1; SAL2, Staphylococcus aureus lipase 3; SHL, Staphylococcus hyicus lipase; BSA, bovine serum albumin; EDTA, ethylene diamine tetraacetic ac
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Expression, purification of a novel alkaline Staphylococcus xylosus lipase acting at high temperature
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 54, Issue 2, 15 April 2011, Pages 93–102
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us