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Recovery and isolation of recombinant human monoclonal antibody from transgenic tobacco plants

Paper ID Volume ID Publish Year Pages File Format Full-Text
3779 188 2011 6 PDF Available
Title
Recovery and isolation of recombinant human monoclonal antibody from transgenic tobacco plants
Abstract

Early steps in the purification process for obtaining recombinant antibody from transgenic plants can significantly affect the overall purity and recovery. We investigate the effects of salt concentration, tissue grinding time and lowering pH on recovery and purification of a recombinant human monoclonal antibody (anti-Pseudomonas aeruginosa serotype O6ad) from transgenic tobacco plants. The presence of 450 mM sodium chloride in the extraction buffer resulted in an 8-fold increase in antibody recovery when compared to using a sodium chloride-free buffer. The grinding time and the pH at which the extract was maintained were also found to have significant effects on antibody recovery. Generally, monoclonal antibody recovery could be enhanced by minimizing its interactions with native tobacco proteins and other components of the tobacco tissue. The overall yield of monoclonal antibody after optimization of the grinding and extraction steps was 30.3 mg/kg of tobacco tissue, this being 12.5 times higher than that at a non-optimized extraction condition.

Research highlights► Transgenic plants offer an alternative to cell culture based production of protein biopharmaceuticals ► Recovery and purification of biopharmaceuticals from transgenic plants is significantly more challenging than their purification from cell culture supernatant primarily due to the extreme complexity of plant extracts ► The early steps in the purification process for obtaining recombinant antibody from transgenic plants can significantly affect the overall purity and recovery. ► Monoclonal antibody recovery could be enhanced by minimizing its interactions with native tobacco proteins and other components of tobacco tissue. ► Parameters such as salt concentration, tissue grinding time and pH affected both recovery and purity of monoclonal antibody.

Keywords
Monoclonal antibody; Transgenic; Tobacco; Recovery; Purification
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Recovery and isolation of recombinant human monoclonal antibody from transgenic tobacco plants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 54, Issue 2, 15 April 2011, Pages 103–108
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us