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Optimising orifice geometry for selective release of periplasmic products during cell disruption by hydrodynamic cavitation

Paper ID Volume ID Publish Year Pages File Format Full-Text
3796 189 2011 3 PDF Available
Title
Optimising orifice geometry for selective release of periplasmic products during cell disruption by hydrodynamic cavitation
Abstract

Downstream processing accounts for a large proportion of the manufacturing cost of biopharmaceutical products. One of the several approaches to improve the process economics include reducing the amount of contaminants and micronised cell debris in the product stream that is presented to the secondary purification steps. We have reported a detailed study on the use of hydrodynamic cavitation and its distinctive capacity to release periplasmic products with higher selectivity compared to other conventional mechanical methods of cell disruption. In the current study, the influence of orifice geometry on selective release of periplasmic proteins is presented. The amount of shear in the system is varied through changing the geometry of the orifice plate, in terms of shape, size and number of orifices used for generating cavities. For a constant intensity of cavitation, of the various shapes studied circular orifice that permits higher number of jet stream from the orifice plates and thus higher shear was found to be advantageous in releasing periplasmic products selectively from E. coli.

Keywords
Cell disruption; Hydrodynamic cavitation; Selective release; Orifice geometry
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Optimising orifice geometry for selective release of periplasmic products during cell disruption by hydrodynamic cavitation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 54, Issue 3, 15 May 2011, Pages 207–209
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us