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Effect of cell spot sizes on micropatterned cultures of rat hepatocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
3812 190 2010 7 PDF Available
Title
Effect of cell spot sizes on micropatterned cultures of rat hepatocytes
Abstract

The effect of cell spot sizes (micropattern sizes) on the morphological and functional behaviors of micropatterned hepatocytes was investigated. Three similar cell chips with collagen spot sizes of 200 μm (chip 200), 500 μm (chip 500), and 800 μm (chip 800) were prepared by the microcontact printing method; the region outside the collagen spots was modified with polyethylene glycol to create the non-adhesion area. The total collagen area of these chips was kept similar. Although the morphology of rat hepatocytes on chips 500 and 800 showed an almost single-cell layer on the collagen spots similar to the random monolayer culture, hepatocytes on chip 200 gathered to the center of the collagen spot and formed multi-cell layers exhibiting a semi-spheroid form. The expression levels of cell–cell interaction molecules (cadherin and connexin-32) and cell function activities (albumin secretion and ammonia removal) were higher in the hepatocytes on chip 200 compared to those in other chip conditions and the random monolayer. These effects may relate to the boundary length obtained from spot size and spot number of collagen, and the cell chip with large boundary region may be superior in maintaining the functional expression of micropatterned hepatocytes.

Keywords
Rat hepatocytes; Micropatterning culture; Collagen; Polyethylene glycol; Liver-specific function; mRNA expression
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Effect of cell spot sizes on micropatterned cultures of rat hepatocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 53, Issue 1, 15 December 2010, Pages 85–91
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us