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An efficient and large-scale preparation process for polysialic acid by Escherichia coli CCTCC M208088

Paper ID Volume ID Publish Year Pages File Format Full-Text
3814 190 2010 7 PDF Available
Title
An efficient and large-scale preparation process for polysialic acid by Escherichia coli CCTCC M208088
Abstract

Polysialic acid (PSA) is a novel pharmaceutical material used in control release for protein drugs or in biomedical applications as scaffold. An efficient pilot production process for bacterial PSA was developed. Our PSA fermentation process by Escherichia coli CCTCC M208088 was optimized in a 500 L fermenter using a novel strategy by controlling pH with ammonia water feeding coupled with sorbitol supplementation. The resulting PSA level increased to 5500 mg/L as compared with the 1500 mg/L of the control. Furthermore, the process for the PSA purification from the fermentation broth was also established. PSA was isolated from the broth by ethanol precipitation, filtration with perlite as filter aid, followed by cetyl pyridinium chloride (CPC) precipitation and lyophilization. The final PSA product obtained had 98.1 ± 1.6% purity at 56.1 ± 1.7% recovery rate. Infrared spectroscopy and NMR spectroscopy analysis indicated that the structure of resulting PSA was identical to the published α-2,8 linked polysialic acid.

Keywords
Escherichia coli; Polysialic acid; Fermentation; Purification
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An efficient and large-scale preparation process for polysialic acid by Escherichia coli CCTCC M208088
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 53, Issue 1, 15 December 2010, Pages 97–103
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us