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A novel thermoactive and alkaline lipase from Talaromyces thermophilus fungus for use in laundry detergents

Paper ID Volume ID Publish Year Pages File Format Full-Text
3816 190 2010 9 PDF Available
Title
A novel thermoactive and alkaline lipase from Talaromyces thermophilus fungus for use in laundry detergents
Abstract

The newly isolated fungus Talaromyces thermophilus was found to exhibit an extracellular lipase activity depending on the growth conditions. A 39 kDa monomeric lipase (TTL) was purified from the culture supernatant using ammonium sulphate precipitation, gel filtration and anion exchange chromatography. TTL N-terminal amino acid sequence showed a high similarity with Thermomyces lanuginosa lipase, a well-known lipase with multiple applications. The specific activity of about 7300 ± 122 and 9868 ± 139 U mg−1 was obtained using tributyrin and olive oil emulsion as substrate, respectively, at pH 9.5 and 50 °C. TTL maximum specific activities were found to be 24,110 ± 390 U mg−1 on trioctanoin. TTL was found to be fairly stable and active on long chain triglycerides at pH 9.5. Interestingly, TTL was found to be resistant to interfacial denaturation since it did not require any detergent to show its maximum activity on pure triglycerides. These findings are particularly important for lipase applications, in particular when variable temperatures and high pH values can be encountered, as well as its ability to show high levels activity in the presence of various surfactants and compatibility with some commercial wash agents and bleach agents.

Keywords
Talaromyces thermophilus lipase; Purification; Characterization; Thermo-alkaline; Detergent
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A novel thermoactive and alkaline lipase from Talaromyces thermophilus fungus for use in laundry detergents
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 53, Issue 1, 15 December 2010, Pages 112–120
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us