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Towards real-time observation of conditioning film and early biofilm formation under laminar flow conditions using a quartz crystal microbalance

Paper ID Volume ID Publish Year Pages File Format Full-Text
3817 190 2010 10 PDF Available
Title
Towards real-time observation of conditioning film and early biofilm formation under laminar flow conditions using a quartz crystal microbalance
Abstract

A real-time quartz crystal microbalance (QCM) technique was employed to monitor early formation of Pseudomonas fluorescens biofilms. To better understand the dynamic process of conditioning film and early biofilm formation, all experiments were conducted in a laminar flow-through chamber under various environmental conditions. Prior to early biofilm formation, a conditioning film comprising organic, inorganic and macromolecular substances was detected over the sensor chip surface within an extremely short duration due to their instantaneous adsorption. Based on atomic force microscopy (AFM) observations, we identified different surface features associated with various conditioning films, demonstrating that the sensor chip surface displayed complex properties in terms of surface topography, roughness, water contact angle, and conditioning film chemistry. There appeared to be a lag time before early biofilm formation. The rate of early biofilm formation was found to differ considerably, depending upon the characteristics of conditioning films and environmental conditions. However, subsequent biofilm formation could be mediated by environmental conditions, reflecting the complex and dynamic process of biofilm development. Of particular interest was the direct in situ real-time observation of the overall sequence of biofilm development processes using a QCM, starting with conditioning film formation, followed by initial bacterial adhesion and subsequently by biofilm formation.

Research highlights▶ The QCM technique has been demonstrated to be a useful tool for direct in situ real-time observation of the overall sequence of biofilm development processes, starting with conditioning film formation, followed by initial bacterial adhesion and subsequently by biofilm formation. ▶ Early biofilm formation may be facilitated through calcium-cation bridging or adsorption via polysaccharide-related compounds on the cell surface. ▶ Conditioning film may play a vital role in initial bacterial attachment due to the accumulated nutrient-rich environment and the conditioning film-induced modification of substratum. ▶ However, subsequent biofilm formation could be mediated by environmental conditions, reflecting the complex and dynamic process of biofilm development.

Keywords
Quartz crystal microbalance; Conditioning film; Early biofilm formation; Laminar flow-through chamber; Pseudomonas fluorescens; Environmental conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 53, Issue 1, 15 December 2010, Pages 121–130
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us