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Time dependent formation of gold nanoparticles in yeast cells: A comparative study

Paper ID Volume ID Publish Year Pages File Format Full-Text
3836 192 2011 6 PDF Available
Title
Time dependent formation of gold nanoparticles in yeast cells: A comparative study
Abstract

Two different strains of yeast, Saccharomyces cerevisiae, AP22 and CCFY-100 were studied for bioaccumulation of gold in the form of H198AuCl4. Thin sectioning and subsequent study by transmission electron microscopy (TEM) reveals that Au3+ was in situ reduced to Au(0) and nano sized gold particles were formed inside the cell. Very low dose γ-energy being responsible for reduction of cationic gold in the polymeric cytoplasm matrix. The formation and entry of gold nanoparticles in the yeast cells were studied as a function of time at certain intervals starting from 15 min to 72 h. The gold nanoparticles gradually moved inward as a function of time from cell wall to cytoplasm to nucleus and finally accumulated in the nucleolus of the cell. TEM image of budding yeast shows that gold nanoparticles are not transferred to the new generation yeast cells.

► This paper for the first time addresses the migration kinetics of nanoparticles inside a cell. ► Gold nanoparticles gradually move inward as a function of time from cell wall to cytoplasm to nucleus. ► The gamma energy of 198Au is solely responsible factor in the reduction of Au and formation of gold nanoparticles. ► TEM image of budding yeast shows that gold nanoparticles are not transferred to the new generation yeast cells.

Keywords
Saccharomyces cerevisiae; TEM; Gold nano particles; H198AuCl4; Thin sectioning; Uptake kinetics
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Time dependent formation of gold nanoparticles in yeast cells: A comparative study
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 55, Issue 1, 15 June 2011, Pages 1–6
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us