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The identity of the cell adhesive protein substrate affects the efficiency of adeno-associated virus reverse transduction

Paper ID Volume ID Publish Year Pages File Format Full-Text
384 40 2012 7 PDF Available
Title
The identity of the cell adhesive protein substrate affects the efficiency of adeno-associated virus reverse transduction
Abstract

Delivering genes from surfaces, called substrate-mediated gene delivery or reverse transduction, is a useful method to achieve spatial localization of gene delivery. We tested the compatibility of adeno-associated virus (AAV) vectors with various cell adhesive proteins to mediate gene delivery from surfaces. Our studies demonstrate that AAV vectors can be successfully adsorbed on collagen I, elastin, and laminin substrates leading to robust gene delivery to overlying cells. Notably, AAV immobilization on laminin yields the highest efficiency of gene expression. This increased gene expression cannot be explained by increases in the levels of virus deposition, transcriptional activity of cells, or virus vector uptake into cells. Further refinement of our knowledge of AAV interactions with extracellular matrix proteins may have important implications in a variety of applications ranging from tissue engineering to in vivo gene therapy.

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Keywords
Adeno-associated virus; Reverse transduction; Gene delivery; Protein adsorption; Micropatterning
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The identity of the cell adhesive protein substrate affects the efficiency of adeno-associated virus reverse transduction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 8, Issue 11, November 2012, Pages 4073–4079
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us