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Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
3847 194 2010 7 PDF Available
Title
Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix
Abstract

A thorough investigation of sucrose hydrolysis by invertase immobilized in recently developed PVA–alginate beads was performed. The developed PVA–alginate bead is distinguished by the treatment with sodium sulfate solution. The performance of invertase immobilized in PVA–alginate matrix beads in terms of leakage, reusability, ideal temperature and pH and kinetic parameters Km and Vmax was investigated. Results revealed that the ideal temperature and pH for the immobilized invertase are 50 °C and 4 respectively. The apparent Km of each bead is relatively lower than that of the free enzyme with the value of 4.7 × 10−5 mM and 2.849 × 10−5 mM for 12PVA–5BA and 12PVA–7BA, respectively. An increase in Vmax was also observed with the value of 2281.02 U mg−1 enzyme and 2281.02 U mg−1 enzyme for 12PVA–5BA and 12PVA–7BA, respectively. The reusability of the beads and its storage period were also investigated and were found to be 14 cycles and 60 days, respectively. Finally, the beads with the best performance (12PVA–5BA) were used to hydrolyze liquid pineapple waste. The production yield of glucose was about 745% in 3 h in the hydrolysis of the liquid pineapple waste.

Keywords
PVA–alginate beads; Sodium sulfate; Invertase kinetic parameter; Pineapple waste
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Hydrolysis of liquid pineapple waste by invertase immobilized in PVA–alginate matrix
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 50, Issue 3, 15 July 2010, Pages 83–89
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us