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Evaluation of mycophenolic acid production by Penicillium brevicompactum MUCL 19011 in batch and continuous submerged cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
3849 194 2010 5 PDF Available
Title
Evaluation of mycophenolic acid production by Penicillium brevicompactum MUCL 19011 in batch and continuous submerged cultures
Abstract

Mycophenolic acid (MPA) is a secondary metabolite produced by Penicillium brevicompactum, which has antibiotic and immunosuppressive properties. In this study, production of MPA by P. brevicompactum MUCL 19011 in shake flask and a 2 L bench-scale stirred tank bioreactor under batch and continuous culture conditions was evaluated. The maximum concentration of MPA, its yield and rate of production in continuous culture were 1.46 g L−1, 29 mg g−1 glucose and 6.8 mg L−1 h−1, respectively. Growth kinetics in batch culture was investigated with five models of Monod, Moser, Verhulst, Contois and Exponential. The compatibility of the experimental data fitted well with the Contois model and the regression value was 0.98. The maximum specific growth rate and saturation constant were 0.07 h−1 and 0.61 g L−1, respectively. Glucose, biomass and MPA concentration profiles were evaluated during the course of fermentation. Under steady-state conditions, glucose consumption, biomass formation and MPA production fluxes were 9.2, 22.35 and 0.194 mmol L−1 h−1, respectively. In batch culture, enzymatically hydrolyzed casein and glycine increased MPA production by 165 and 112%, respectively.

Keywords
Mycophenolic acid; Penicillium brevicompactum; Continuous culture; Growth kinetics; Submerged; Stirred tank
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Evaluation of mycophenolic acid production by Penicillium brevicompactum MUCL 19011 in batch and continuous submerged cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 50, Issue 3, 15 July 2010, Pages 99–103
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us