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Bioprocess design and economics of recombinant BM86/BM95 antigen production for anti-tick vaccines

Paper ID Volume ID Publish Year Pages File Format Full-Text
3872 196 2010 12 PDF Available
Title
Bioprocess design and economics of recombinant BM86/BM95 antigen production for anti-tick vaccines
Abstract

Cattle ticks, Rhipicephalus (Boophilus) spp., infestations are a major veterinary problem that impacts cattle production worldwide. Recently, vaccination has emerged as an alternative to control tick infestations that has shown the advantage of being cost-effective, avoids environmental contamination and prevents selection of drug-resistant ticks resulting from repeated acaricides applications. However, the success of vaccination is highly dependent on the availability of effective vaccines at affordable prices. Recombinant DNA technology has provided the mean for producing antigens in large quantities making possible the development and commercialization of anti-tick vaccines. In the early 1990s, a technology was developed to produce a commercial anti-tick vaccine based on the recombinant BM86 (rBM86) antigen isolated from R. (Boophilus) microplus and intracellularly expressed in Pichia pastoris. However, the technological process for its production was expensive. Herein we conducted an economical analysis of this technology in comparison with other reported processes. The results allowed the identification of critical steps in the technology and addressed research for bioprocess optimization. As a result, two alternative bioprocesses were developed to produce anti-tick vaccines based on the rBM86 protein secreted in P. pastoris and by displaying antigenic peptides on the Escherichia coli membrane (BM95-MSP1a antigen). Vaccine production costs were reduced from US$0.92/dose for the intracellular rBM86 to US$0.72/dose and US$0.56/dose for the secreted rBM86 and BM95-MSP1a, respectively.

Keywords
Tick; Pichia pastoris; Boophilus microplus; Bm86; Bm95; Bioprocess economics
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Bioprocess design and economics of recombinant BM86/BM95 antigen production for anti-tick vaccines
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 52, Issue 1, 15 October 2010, Pages 79–90
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us