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Tissular model/sensor seamless system for qualified analysis and its characterization

Paper ID Volume ID Publish Year Pages File Format Full-Text
3876 196 2010 6 PDF Available
Title
Tissular model/sensor seamless system for qualified analysis and its characterization
Abstract

Clinical drug discovery in most cases begins with molecular screening in order to select a lead-substance. This process is a key step in successful drug development. Lead-substances are marked based on their ability to affect objective biological properties. In order to judge the molecular efficacy of a lead-substance, both animal experimentation and cell-based bioassay have been employed. However, in high throughput assay, cellular biosensing is one of the smart methods. In this study, an NO sensor device was developed which has two functions: NO sensing in cell culture media, and cell adhesion for mammalian cell cultivation. Endothelium cells with intact functionality were also investigated on the molecular level as a tissular model. Furthermore, we analyzed the response graph of the sensor output from the cellular NO sensor. We conclude that, using the multivariate data analysis method, of principal component analysis (PCA), it is possible to deduce typical biological events from the sensor output.

Keywords
Qualified analysis; Cellular biosensing; Nitric oxide; High throughput analysis (HTA); Electrochemical sensing; Multivariate data analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 52, Issue 1, 15 October 2010, Pages 110–115
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us