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Optimizing l-(+)-lactic acid production by thermophile Lactobacillus plantarum As.1.3 using alternative nitrogen sources with response surface method

Paper ID Volume ID Publish Year Pages File Format Full-Text
3893 197 2010 8 PDF Available
Title
Optimizing l-(+)-lactic acid production by thermophile Lactobacillus plantarum As.1.3 using alternative nitrogen sources with response surface method
Abstract

The effects of five alternative nitrogen sources, namely, malt sprout (MS), corn steep liquor (CSL), NH4Cl, NH4NO3 and diamine citrate (DC) were investigated on the l-(+)-lactic acid (LA) production by thermophile Lactobacillus plantarum As.1.3. Through the statistical analysis of the results by three steps of response surface methodology (RSM) design, MS and CSL were found to have significant effects on the LA production and their optimal concentrations in the medium should be 16.0 g/L and 12.0 g/L, respectively. The verification of the optimized medium showed that the maximum specific growth rate (μm) was 1.09 h−1, the cell yield coefficient (YX/S) and the l-(+)-lactic acid yield coefficient (YP/S) were 0.233 (OD620/g) and 0.98 (g/g), and the maximum volumetric productivity and the average volumetric productivity were 13.0 g/L h and 3.20 g/L h, respectively. The results indicate that the LA production can also be enhanced with the inexpensive nitrogen source alternatives.

Keywords
LA, l-(+)-lactic acid; YE, yeast extract; E, beef extract; MS, malt sprout; CSL, corn steep liquor; RSM, response surface designl-(+)-Lactic acid; Thermophile Lactobacillus plantarum As.1.3; Malt sprout; Corn steep liquor; Response surface methodology; Ni
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Optimizing l-(+)-lactic acid production by thermophile Lactobacillus plantarum As.1.3 using alternative nitrogen sources with response surface method
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 52, Issues 2–3, 15 November 2010, Pages 212–219
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us